6PGL
Structure of Kluyveromyces marxianus Usb1 with uridine monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-17 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9787 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.957, 110.344, 114.697 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.172 - 1.845 |
R-factor | 0.1894 |
Rwork | 0.188 |
R-free | 0.21940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.987 |
Data scaling software | STARANISO |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.520 | 2.040 |
High resolution limit [Å] | 1.840 | 1.840 |
Number of reflections | 82782 | 2827 |
<I/σ(I)> | 15.3 | |
Completeness [%] | 94.9 | |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.2 M potassium sodium tartrate, 100 mM HEPES, 30% glycerol |