6P95

Structure of Lassa virus glycoprotein in complex with Fab 25.6A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-07-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033188
Spacegroup name	P 61 2 2
Unit cell lengths	152.242, 152.242, 453.889
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.660 - 3.500
R-factor	0.2072
Rwork	0.205
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vk2
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.982	3.640
High resolution limit [Å]	3.500	3.500
Rmerge	0.148	1.429
Rmeas	0.153	1.486
Rpim	0.041	0.404
Number of reflections	40281	4448
<I/σ(I)>	17.4
Completeness [%]	99.8	100
Redundancy	13.3	13.3
CC(1/2)	0.999	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1 M Tris pH 8, 15-18% PEG 3350 and 0.1 M-0.3 M magnesium acetate