6P95
Structure of Lassa virus glycoprotein in complex with Fab 25.6A
Summary for 6P95
| Entry DOI | 10.2210/pdb6p95/pdb |
| Related | 6P91 |
| Descriptor | Pre-glycoprotein polyprotein GP complex, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total) |
| Functional Keywords | lassa virus, glycoprotein, antibody, viral protein, viral protein-immune system complex, viral protein/immune system |
| Biological source | Lassa virus (LASV) More |
| Total number of polymer chains | 12 |
| Total formula weight | 305481.89 |
| Authors | Saphire, E.O.,Hastie, K.M. (deposition date: 2019-06-09, release date: 2019-09-11, Last modification date: 2024-10-30) |
| Primary citation | Hastie, K.M.,Cross, R.W.,Harkins, S.S.,Zandonatti, M.A.,Koval, A.P.,Heinrich, M.L.,Rowland, M.M.,Robinson, J.E.,Geisbert, T.W.,Garry, R.F.,Branco, L.M.,Saphire, E.O. Convergent Structures Illuminate Features for Germline Antibody Binding and Pan-Lassa Virus Neutralization. Cell, 178:1004-1015.e14, 2019 Cited by PubMed Abstract: Lassa virus (LASV) causes hemorrhagic fever and is endemic in West Africa. Protective antibody responses primarily target the LASV surface glycoprotein (GPC), and GPC-B competition group antibodies often show potent neutralizing activity in humans. However, which features confer potent and broadly neutralizing antibody responses is unclear. Here, we compared three crystal structures of LASV GPC complexed with GPC-B antibodies of varying neutralization potency. Each GPC-B antibody recognized an overlapping epitope involved in binding of two adjacent GPC monomers and preserved the prefusion trimeric conformation. Differences among GPC-antibody interactions highlighted specific residues that enhance neutralization. Using structure-guided amino acid substitutions, we increased the neutralization potency and breadth of these antibodies to include all major LASV lineages. The ability to define antibody residues that allow potent and broad neutralizing activity, together with findings from analyses of inferred germline precursors, is critical to develop potent therapeutics and for vaccine design and assessment. PubMed: 31398326DOI: 10.1016/j.cell.2019.07.020 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.5 Å) |
Structure validation
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