6P67

Crystal Structure of a Complex of human IL-7Ralpha with an anti-IL-7Ralpha 2B8 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	84.900, 219.680, 90.350
Unit cell angles	90.00, 104.33, 90.00

Refinement procedure

Resolution	43.770 - 2.900
R-factor	0.2491
Rwork	0.249
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3di3 for IL-7R and 6P4Y for 2B8
RMSD bond length	0.003
RMSD bond angle	0.660
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	phenix.refine (1.16rc1_3535)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.004
High resolution limit [Å]	2.900	2.900
Rmeas	0.196
Number of reflections	70309	6971
<I/σ(I)>	8.41	1.08
Completeness [%]	99.2	98.72
Redundancy	5.8	5.8
CC(1/2)	0.996	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	17% PEG 3350, 10% MPD, 0.2 lithium sulfate, 0.1 M imidazole pH 6.5, 20% glycerol