6P50

Crystal Structure of a Complex of human IL-7Ralpha with an anti-IL-7Ralpha Fab 4A10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	C 1 2 1
Unit cell lengths	145.113, 40.171, 105.097
Unit cell angles	90.00, 105.48, 90.00

Refinement procedure

Resolution	38.610 - 2.900
R-factor	0.2879
Rwork	0.285
R-free	0.32980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3DI3 for IL-7R chain and 6P4Y for 4A10
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASES
Refinement software	PHENIX (1.16rc1_3535)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.610	3.070
High resolution limit [Å]	2.900	2.900
Rmeas	0.283	1.140
Number of reflections	12886	1892
<I/σ(I)>	3.63	1.02
Completeness [%]	96.3	89.2
Redundancy	2.9	2.5
CC(1/2)	0.969	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	25% PEG 3350, 0.2 M sodium malonate pH 6.0, 20% glycerol