6P4Y

Crystal Structure of anti-IL-7Ralpha 4A10 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03317
Spacegroup name	P 1 21 1
Unit cell lengths	37.372, 78.496, 69.480
Unit cell angles	90.00, 96.23, 90.00

Refinement procedure

Resolution	39.248 - 1.799
R-factor	0.1897
Rwork	0.188
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ae6
RMSD bond length	0.006
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.15_3448: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.248	1.900
High resolution limit [Å]	1.799	1.799
Rmerge	0.126	1.418
Rmeas	0.149	1.418
Rpim	0.080	0.921
Number of reflections	36735	3613
<I/σ(I)>	8.33	0.96
Completeness [%]	99.1	96.46
Redundancy	3.3	3.2
CC(1/2)	0.994	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	30% PEG 5000, 0.1 M sodium acetate, 20% glycerol, seeded