6OTC

Synthetic Fab bound to Marburg virus VP35 interferon inhibitory domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	57.827, 97.422, 110.325
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.960 - 1.700
R-factor	0.1976
Rwork	0.196
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4GHL & 1TZI
RMSD bond length	0.006
RMSD bond angle	1.307
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.960	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	68713	3348
<I/σ(I)>	15.8
Completeness [%]	99.4
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	10 mM Bis-Tris, pH 6.5, 20% PEG5000 MME