6OSP
Crystal Structure Analysis of PIP4K2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.190, 98.680, 105.120 |
| Unit cell angles | 90.00, 93.66, 90.00 |
Refinement procedure
| Resolution | 44.648 - 2.210 |
| R-factor | 0.1798 |
| Rwork | 0.177 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ybx |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.320 | 46.330 | 2.250 |
| High resolution limit [Å] | 2.210 | 5.990 | 2.210 |
| Rmeas | 0.079 | 0.025 | 1.181 |
| Rpim | 0.041 | 0.013 | 0.606 |
| Total number of observations | 168656 | 8444 | 8280 |
| Number of reflections | 44869 | 2318 | 2193 |
| <I/σ(I)> | 13.1 | 40 | 1.3 |
| Completeness [%] | 99.5 | 99.6 | 99 |
| Redundancy | 3.8 | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, 200mM Malic acid |
