6OPA
Crystal structure of bovine Fab NC-Cow1 in complex with HIV-1 BG505 SOSIP.664, and human Fabs 35022 and PGT128
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 215.244, 215.244, 438.798 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.755 - 4.084 |
| R-factor | 0.3328 |
| Rwork | 0.332 |
| R-free | 0.34310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cez 4toy 3tv3 6oo0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.940 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.900 | 4.170 |
| High resolution limit [Å] | 4.080 | 4.080 |
| Rmeas | 0.232 | 2.280 |
| Rpim | 0.076 | 0.760 |
| Number of reflections | 30902 | 1515 |
| <I/σ(I)> | 11.2 | 0.34 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 8.9 |
| CC(1/2) | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.56 | 298 | 0.2M sodium citrate, 0.1M Tris, 30% Peg400 |
