6OD5

Human TCF4 C-terminal bHLH domain in Complex with 12-bp Oligonucleotide Containing E-box Sequence with 5-carboxylcytosines

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	44.677, 44.757, 54.640
Unit cell angles	78.63, 78.88, 79.27

Refinement procedure

Resolution	36.661 - 2.050
R-factor	0.2364
Rwork	0.234
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ql2
RMSD bond length	0.003
RMSD bond angle	0.552
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.661	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.263	0.554
Rpim	0.138	0.412
Number of reflections	24281	2094
<I/σ(I)>	5.4
Completeness [%]	96.5	82.8
Redundancy	3.6	2.2
CC(1/2)		0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	30% PEG4000, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate monohydrate or 0.2 M sodium acetate trihydrate