6OD4
Human TCF4 C-terminal bHLH domain in Complex with 11-bp Oligonucleotide Containing E-box Sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.614, 43.602, 43.557 |
| Unit cell angles | 97.17, 102.56, 102.53 |
Refinement procedure
| Resolution | 41.817 - 1.699 |
| R-factor | 0.2242 |
| Rwork | 0.222 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ql2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.423 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.820 | 1.750 |
| High resolution limit [Å] | 1.699 | 1.700 |
| Rmerge | 0.103 | 0.711 |
| Rpim | 0.041 | 0.418 |
| Number of reflections | 26021 | 2269 |
| <I/σ(I)> | 16.4 | 3.7 |
| Completeness [%] | 95.3 | 83.1 |
| Redundancy | 6.2 | 3.2 |
| CC(1/2) | 0.522 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 20% PEG4000, 10% 2-propanol, 0.1 M sodium citrate tribasic dihydrate, pH 5.6 |
