6OD4

Human TCF4 C-terminal bHLH domain in Complex with 11-bp Oligonucleotide Containing E-box Sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1
Unit cell lengths	36.614, 43.602, 43.557
Unit cell angles	97.17, 102.56, 102.53

Refinement procedure

Resolution	41.817 - 1.699
R-factor	0.2242
Rwork	0.222
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ql2
RMSD bond length	0.002
RMSD bond angle	0.423
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.820	1.750
High resolution limit [Å]	1.699	1.700
Rmerge	0.103	0.711
Rpim	0.041	0.418
Number of reflections	26021	2269
<I/σ(I)>	16.4	3.7
Completeness [%]	95.3	83.1
Redundancy	6.2	3.2
CC(1/2)		0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	292	20% PEG4000, 10% 2-propanol, 0.1 M sodium citrate tribasic dihydrate, pH 5.6