6OAU

Apo Structure of WT Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D produced in GnTI-deficient HEK293-F cells

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	177.480, 97.250, 77.210
Unit cell angles	90.00, 93.47, 90.00

Refinement procedure

Resolution	35.500 - 2.480
R-factor	0.215
Rwork	0.213
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lpa
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BUSTER (2.11.7)
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.580	88.580	2.620
High resolution limit [Å]	2.480	7.850	2.480
Rmerge	0.125	0.105	0.546
Rmeas	0.148	0.126	0.651
Rpim	0.080	0.069	0.352
Total number of observations	154530
Number of reflections	45839	1459	6706
<I/σ(I)>	6.5
Completeness [%]	98.8	95.1	99.9
Redundancy	3.4	3.2	3.3
CC(1/2)	0.978	0.963	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M Sodium Malonate, 20% PEG3350, 4% 2-propanol