6O5F
Crystal structure of DEAD-box RNA helicase DDX3X at pre-unwound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-13 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 66.102, 66.102, 230.463 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.902 - 2.504 |
| R-factor | 0.2127 |
| Rwork | 0.212 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e7i |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.700 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.092 | |
| Number of reflections | 37345 | |
| <I/σ(I)> | 8.8 | 0.9 |
| Completeness [%] | 96.4 | 99 |
| Redundancy | 3.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 25% (v/v) PEG 3350, 0.2 M magnesium chloride, 0.1 M Tris pH 8.5 |
