6O5F

Crystal structure of DEAD-box RNA helicase DDX3X at pre-unwound state

Summary for 6O5F

• Entry DOI: 10.2210/pdb6o5f/pdb
• Descriptor: ATP-dependent RNA helicase DDX3X, RNA (5'-R(P*CP*AP*AP*GP*GP*UP*CP*AP*UP*UP*CP*GP*CP*AP*AP*GP*AP*GP*UP*GP*GP*CP*C)-3'), CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: ddx3x, dead-box, rna helicase, hydrolase, hydrolase-rna complex, hydrolase/rna
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 125672.54

Authors

Song, H.,Ji, X. (deposition date: 2019-03-02, release date: 2019-07-24, Last modification date: 2023-10-11)

Primary citation

Song, H.,Ji, X.
The mechanism of RNA duplex recognition and unwinding by DEAD-box helicase DDX3X.
Nat Commun, 10:3085-3085, 2019

PubMed Abstract: DEAD-box helicases (DDXs) regulate RNA processing and metabolism by unwinding short double-stranded (ds) RNAs. Sharing a helicase core composed of two RecA-like domains (D1D2), DDXs function in an ATP-dependent, non-processive manner. As an attractive target for cancer and AIDS treatment, DDX3X and its orthologs are extensively studied, yielding a wealth of biochemical and biophysical data, including structures of apo-D1D2 and post-unwound D1D2:single-stranded RNA complex, and the structure of a D2:dsRNA complex that is thought to represent a pre-unwound state. However, the structure of a pre-unwound D1D2:dsRNA complex remains elusive, and thus, the mechanism of DDX action is not fully understood. Here, we describe the structure of a D1D2 core in complex with a 23-base pair dsRNA at pre-unwound state, revealing that two DDXs recognize a 2-turn dsRNA, each DDX mainly recognizes a single RNA strand, and conformational changes induced by ATP binding unwinds the RNA duplex in a cooperative manner.

PubMed: 31300642
DOI: 10.1038/s41467-019-11083-2

Experimental method

X-RAY DIFFRACTION (2.504 Å)