6O3C
Crystal structure of active Smoothened bound to SAG21k, cholesterol, and NbSmo8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 293.15 |
Detector technology | PIXEL |
Collection date | 2019-01-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 39.010, 121.090, 157.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.270 | 1.870 |
Number of reflections | 18464 | 1732 |
<I/σ(I)> | 5.48 | 0.92 |
Completeness [%] | 96.2 | 94 |
Redundancy | 6.63 | 3.5 |
CC(1/2) | 0.992 | 0.230 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293.15 | 0.1 M MES pH 6.0, 0.1 M ammonium phosphate dibasic, 1.5% 1,2,3-Heptanetriol, 22% PEG 300 |