6O03

Monobody (MC17) bound to tyrosine kinase binding domain of E3 ubiquitin ligase CBL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-30
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	87.104, 67.156, 167.882
Unit cell angles	90.00, 96.22, 90.00

Refinement procedure

Resolution	46.190 - 3.300
R-factor	0.2891
Rwork	0.285
R-free	0.32970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.802
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.490	3.418
High resolution limit [Å]	3.300	3.300
Number of reflections	14407
<I/σ(I)>	8.66
Completeness [%]	98.0
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% (w/v) PEG 3000, 100mM tris base/hydrochloric acid pH 7, 200mM calcium acetate