6NQ3
Crystal Structure of a SUZ12-RBBP4-PHF19-JARID2 Heterotetrameric Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2016-06-18 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 127.710, 139.600, 268.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.600 - 2.890 |
R-factor | 0.173 |
Rwork | 0.170 |
R-free | 0.23000 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.110 | 2.950 |
High resolution limit [Å] | 2.890 | 2.890 |
Number of reflections | 52773 | |
<I/σ(I)> | 17.3 | |
Completeness [%] | 98.9 | |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% polyethylene glycol (PEG) 3350, 0.1M Sodium Malonate, pH6.0 |