6NMB

Tranexamic Acid is an Active Site Inhibitor of Urokinase Plasminogen Activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	CCD
Collection date	2017-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	73.043, 65.446, 111.136
Unit cell angles	90.00, 97.42, 90.00

Refinement procedure

Resolution	48.560 - 2.300
R-factor	0.2233
Rwork	0.221
R-free	0.27120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vnt
RMSD bond length	0.017
RMSD bond angle	1.845
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.560	2.420
High resolution limit [Å]	2.300	2.300
Rmeas	0.226	0.971
Rpim	0.087	0.439
Number of reflections	42244
<I/σ(I)>	5.3
Completeness [%]	90.0
Redundancy	6.6
CC(1/2)	0.995	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.03 M sodium nitrate, 0.03 M sodium phosphate, 0.03 M ammonium sulfate, 0.1 M Tris bicine, 25% v/v PEG500, 10% v/v PEG20000