6NJT

Mouse endonuclease G mutant - H97A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-06-17
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	P 31 2 1
Unit cell lengths	109.609, 109.609, 118.062
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.710 - 2.070
R-factor	0.1854
Rwork	0.184
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gkp
Data reduction software	XDS
Data scaling software	Aimless (v0.7.1)
Phasing software	PHASER (v2.7.16)
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.800	2.130
High resolution limit [Å]	2.070	2.070
Rmerge	0.277	2.691
Rpim	0.145	1.618
Number of reflections	50410	3865
<I/σ(I)>	8.2
Completeness [%]	99.9	99.9
Redundancy	8.8	7.1
CC(1/2)	0.992	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	290	25% isopropanol, 0.2 M MgCl2, 0.1 M HEPES pH 7.6, 0.75 mM G(5HMC) dinucleotide