6NFJ
Structure of Beta-Klotho in Complex with FGF19 C-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.090, 143.900, 141.050 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 59.420 - 3.190 |
| R-factor | 0.2815 |
| Rwork | 0.280 |
| R-free | 0.31960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vaq |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.446 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.420 | 3.310 |
| High resolution limit [Å] | 3.190 | 3.200 |
| Number of reflections | 42869 | |
| <I/σ(I)> | 3.4 | 0.81 |
| Completeness [%] | 99.2 | 95.87 |
| Redundancy | 3.8 | 3.3 |
| CC(1/2) | 0.519 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 21% PEG1500, 0.1M Ammonium sulphate, 0.2M NDSB-201, 0.1M Tris-HCl pH 7.5 |
