6NCM

Crystal structure of the human FOXN3 DNA binding domain in complex with a forkhead-like (FHL) DNA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	42.540, 72.310, 102.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.300 - 2.704
R-factor	0.2377
Rwork	0.234
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nce
RMSD bond length	0.003
RMSD bond angle	0.484
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	2.801
High resolution limit [Å]	2.704	2.704
Rmerge	0.090	1.949
Rmeas	0.099	2.115
Rpim	0.039
Number of reflections	9108	873
<I/σ(I)>	11.66	0.85
Completeness [%]	99.2	98.87
Redundancy	6.5	6.6
CC(1/2)	0.999	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.1 M BisTris pH 5.5, 0.2 M NaCl, 22% PEG 3350