6MXD
Crystal structure of Trypanosoma brucei hypoxanthine-guanine-xanthine phosphoribosyltranferase in complex with IMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-06 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.285, 64.984, 79.075 |
| Unit cell angles | 85.74, 74.58, 62.87 |
Refinement procedure
| Resolution | 47.270 - 2.960 |
| R-factor | 0.257 |
| Rwork | 0.252 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tc2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.577 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.268 | 3.140 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Rmerge | 0.148 | 0.397 |
| Number of reflections | 20692 | 6539 |
| <I/σ(I)> | 3.5 | 1.3 |
| Completeness [%] | 98.6 | 97.3 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 25% PEG 3350, 0.2 M lithium sulphate and 0.1 M Bis-Tris pH 5 |
