6MWG
NavAb Voltage-gated Sodium Channel, residues 1-239, with mutation T206V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 124.175, 124.175, 189.868 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.770 - 2.501 |
R-factor | 0.2211 |
Rwork | 0.220 |
R-free | 0.23890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rvy |
RMSD bond length | 0.007 |
RMSD bond angle | 0.915 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 25875 | |
<I/σ(I)> | 24.6 | |
Completeness [%] | 99.8 | |
Redundancy | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 1.8 M Ammonium Sulfate 100 mM Sodium Acetate, pH 5.8 |