6MPX

Twelve chloride ions induce formation and stabilize the NC1 hexamer of collagen IV assembled from transition state trimers

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-16
Detector	RAYONIX MX-300
Wavelength(s)	0.9786
Spacegroup name	P 41 21 2
Unit cell lengths	121.160, 121.160, 106.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.893 - 1.900
R-factor	0.1492
Rwork	0.148
R-free	0.17670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1li1
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	85.673	40.387	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge		0.061	0.443
Rmeas	0.129	0.065	0.470
Rpim	0.041	0.021	0.154
Total number of observations	558146
Number of reflections	62515	2152	9000
<I/σ(I)>	9.5	9.8	1.7
Completeness [%]	99.9	98.7	100
Redundancy	8.9	8.1	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	.085 M HEPES (pH 7.5), 1.68 M ammonium sulfate, and 1.5% (w/v) PEG 400