6MNH
ULK1 Unc-51 like autophagy activating kinase in complex with inhibitor BTC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-13 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 101.984, 44.884, 58.022 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.140 - 1.730 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.22780 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.191 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.140 | 1.820 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.065 | 0.425 |
Number of reflections | 28537 | 4090 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 99.1 | 99.7 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 281 | 31% PEG 4K, 200mM calcium chloride, 100mM bis tris propane pH 8.5 |