6MLB
Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:K40L:T51F mutant of human cellular retinol binding protein II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-05 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.476, 63.282, 117.782 |
| Unit cell angles | 90.00, 95.94, 90.00 |
Refinement procedure
| Resolution | 33.685 - 2.150 |
| R-factor | 0.2316 |
| Rwork | 0.228 |
| R-free | 0.30240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rct |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.546 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.685 | 2.227 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.100 | 0.840 |
| Rmeas | 0.112 | |
| Rpim | 0.348 | |
| Number of reflections | 28616 | 2760 |
| <I/σ(I)> | 32.2 | |
| Completeness [%] | 98.0 | 96.6 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, ammonium acetate, sodium acetate |
