6MF2

Improved Model of Human Coagulation Factor VIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	134.569, 134.569, 359.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.365 - 3.609
R-factor	0.253947279805
Rwork	0.252
R-free	0.28356
RMSD bond length	0.005
RMSD bond angle	1.149
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.365	3.738
High resolution limit [Å]	3.609	3.609
Number of reflections	31887
<I/σ(I)>	0.7
Completeness [%]	81.7
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	8-10% PEG8000, 0.1 M imidazole, 100-300 mM sodium chloride