6MF0

Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	80
Detector technology	CCD
Collection date	2014-06-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.977408
Spacegroup name	P 1 21 1
Unit cell lengths	72.005, 135.863, 196.107
Unit cell angles	90.00, 90.15, 90.00

Refinement procedure

Resolution	49.027 - 3.200
R-factor	0.209883646983
Rwork	0.206
R-free	0.28740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r7e
RMSD bond length	0.011
RMSD bond angle	1.462
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.027	3.250
High resolution limit [Å]	3.198	3.200
Rmerge	0.110	0.648
Rmeas	0.155	0.917
Rpim	0.110	0.648
Number of reflections	62358
<I/σ(I)>	7.3
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.987	0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	16% PEG3350, 0.025 M magnesium chloride, 0.025 M HEPES