6MF0
Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-06-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.977408 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.005, 135.863, 196.107 |
| Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
| Resolution | 49.027 - 3.200 |
| R-factor | 0.209883646983 |
| Rwork | 0.206 |
| R-free | 0.28740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r7e |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.462 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.027 | 3.250 |
| High resolution limit [Å] | 3.198 | 3.200 |
| Rmerge | 0.110 | 0.648 |
| Rmeas | 0.155 | 0.917 |
| Rpim | 0.110 | 0.648 |
| Number of reflections | 62358 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 2 | |
| CC(1/2) | 0.987 | 0.542 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG3350, 0.025 M magnesium chloride, 0.025 M HEPES |
