6MEK

Crystal structure of Hepatitis C virus envelope glycoprotein E2 core in complex with human antibodies HEPC3 and HEPC46

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	75.399, 105.589, 337.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.967 - 3.100
R-factor	0.2265
Rwork	0.223
R-free	0.28990
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	89.490	89.490	2.980
High resolution limit [Å]	2.900	12.970	2.900
Rmerge	0.160	0.048	1.632
Rmeas	0.168	0.051	1.745
Rpim	0.052	0.015	0.593
Total number of observations	617678
Number of reflections	60807	807	4367
<I/σ(I)>	10.7
Completeness [%]	99.9	99.8	99.6
Redundancy	10.2	10.6	8.2
CC(1/2)	0.997	0.999	0.411

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.02 M Nickel(II) chloride hexahydrate, 0.02 M magnesium chloride hexahydrate, 0.02 M cadmium chloride hydrate, 0.1 M sodium acetate trihydrate pH 4.5, 24% PEG 2,000