6MEJ

Crystal structure of Hepatitis C virus envelope glycoprotein E2 ectodomain in complex with human antibodies HEPC3 and HEPC46

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	86.440, 76.640, 118.260
Unit cell angles	90.00, 106.33, 90.00

Refinement procedure

Resolution	78.271 - 2.800
R-factor	0.2019
Rwork	0.198
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mwf 6med 6meg
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.270	78.270	2.690
High resolution limit [Å]	2.600	10.070	2.600
Rmerge	0.125	0.049	1.641
Rmeas	0.152	0.059	2.009
Rpim	0.085	0.033	1.144
Total number of observations	142433	2659	13995
Number of reflections	45937	854	4502
<I/σ(I)>	5.6	16.4	0.7
Completeness [%]	99.9	99.8	99.9
Redundancy	3.1	3.1	3.1
CC(1/2)	0.976	0.995	0.258

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	298	0.2 M Ammonium sulfate, 20% Peg 3,350