6MCK
p97 D1D2 with CB5083 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 127.410, 263.830, 164.130 |
| Unit cell angles | 90.00, 103.67, 90.00 |
Refinement procedure
| Resolution | 35.030 - 3.770 |
| R-factor | 0.22448 |
| Rwork | 0.222 |
| R-free | 0.26452 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.289 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.770 | 3.770 |
| Number of reflections | 89790 | |
| <I/σ(I)> | 9.91 | |
| Completeness [%] | 83.9 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.1 M sodium citrate, pH 4.5, 250 mM Tri-sodium citrate and 15 % PEG3350 |
