6MBA

Crystal Structure of Human Nav1.4 CTerminal Domain in Complex with apo Calmodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.918
Spacegroup name	C 1 2 1
Unit cell lengths	112.720, 29.041, 94.991
Unit cell angles	90.00, 123.65, 90.00

Refinement procedure

Resolution	44.875 - 1.799
R-factor	0.2043
Rwork	0.203
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ovn
RMSD bond length	0.016
RMSD bond angle	1.384
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.875	44.500	1.850
High resolution limit [Å]	1.799	8.050	1.800
Rmerge	0.060	0.046	0.429
Rmeas	0.084	0.065	0.591
Number of reflections	42682	452	3101
<I/σ(I)>	6.57	13.37	1.53
Completeness [%]	91.4	85.8	90.6
Redundancy	1.605	1.712	1.581
CC(1/2)	0.991	0.987	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	0.1M MES pH 6.0, 20% PEG 6000, 1.0M LiCl