6MAM

Cleaved Ebola GP in complex with a broadly neutralizing human antibody, ADI-15946

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-07-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 43 2 2
Unit cell lengths	182.273, 182.273, 262.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.544 - 4.100
R-factor	0.2684
Rwork	0.262
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hj3
RMSD bond length	0.002
RMSD bond angle	0.587
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.544	4.300
High resolution limit [Å]	4.100	4.100
Number of reflections	35312	3460
<I/σ(I)>	14.6	1.8
Completeness [%]	99.6	100
Redundancy	13.1	13.4
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.2	295	0.2 M sodium citrate tribasic dihydrate, 20% polyethelyene glycol 3350