6MAK

HBO1 is required for the maintenance of leukaemia stem cells

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953736
Spacegroup name	I 1 2 1
Unit cell lengths	87.628, 39.026, 107.765
Unit cell angles	90.00, 99.59, 90.00

Refinement procedure

Resolution	43.200 - 2.130
R-factor	0.21629
Rwork	0.214
R-free	0.26434
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.204
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.200	2.190
High resolution limit [Å]	2.130	2.130
Rmerge	0.070	0.748
Rpim	0.029	0.310
Number of reflections	19938	1558
<I/σ(I)>	13.1	2.6
Completeness [%]	98.0	94.2
Redundancy	6.9	6.6
CC(1/2)	0.998	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein at 2.5 mg/mL was added in equal volume ratio with reservoir, 200 nL plus 200 nL. The reservoir consisted of 22% PEG MME 2000 plus 57 mM diammonium tartrate. Plates were stored at 20 C.