6M9K

Crystal structure of lambda exonuclease in complex with the Red beta C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-16
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	122.522, 122.522, 147.858
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	106.110 - 2.300
R-factor	0.1982
Rwork	0.195
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1avq
RMSD bond length	0.009
RMSD bond angle	1.271
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	106.110	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.130	0.049	0.580
Rmeas	0.149	0.053	0.747
Rpim	0.071	0.021	0.460
Number of reflections	57486	3065	2822
<I/σ(I)>	6.8
Completeness [%]	99.7	99.3	99.1
Redundancy	3.9	6.1	2.3
CC(1/2)		0.998	0.461

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	PEG3350, LISO4, BIS-TRIS