1AVQ

TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS

Summary for 1AVQ

Entry DOI	10.2210/pdb1avq/pdb
Descriptor	LAMBDA EXONUCLEASE, PHOSPHATE ION, ACETATE ION, ... (4 entities in total)
Functional Keywords	deoxyribonuclease, dna recombination and repair, 5'-3' exonuclease
Biological source	Enterobacteria phage lambda
Total number of polymer chains	3
Total formula weight	79018.52
Authors	Kovall, R.A.,Matthews, B.W. (deposition date: 1997-09-18, release date: 1998-03-18, Last modification date: 2024-02-07)
Primary citation	Kovall, R.,Matthews, B.W. Toroidal structure of lambda-exonuclease. Science, 277:1824-1827, 1997 Cited by PubMed Abstract: Structure determination at 2.4 angstrom resolution shows that lambda-exonuclease consists of three subunits that form a toroid. The central channel is funnel shaped, tapering from an inner diameter of about 30 angstroms at the wider end to 15 angstroms at the narrow end. This is adequate to accommodate the DNA substrate and thus provides a structural basis for the ability of the enzyme to sequentially hydrolyze thousands of nucleotides in a highly processive manner. The results also suggest the locations of the active sites and the constraints that limit cleavage to a single strand. PubMed: 9295273 DOI: 10.1126/science.277.5333.1824 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.4 Å)

Structure validation