6M93

Monophosphorylated pSer33 b-Catenin peptide, b-TrCP/Skp1, NRX-1933 ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-27
Detector	RAYONIX MX-300
Wavelength(s)	0.9795
Spacegroup name	P 31
Unit cell lengths	82.530, 82.530, 111.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.265 - 2.500
R-factor	0.2147
Rwork	0.213
R-free	0.23000
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.265	41.265	2.570
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.115	0.093	1.940
Rmeas	0.134	0.111	2.201
Number of reflections	29168	287	2173
<I/σ(I)>	10.91	23.08	0.94
Completeness [%]	99.5	86.4	100
Redundancy	4.115	3.3	4.436
CC(1/2)	0.991	0.974	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8% PEG 4000 0.1M BTP pH 5.5