6M4V
Crystal structure of MBP fused split FKBP in complex with rapamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-11-20 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.026, 48.144, 108.730 |
| Unit cell angles | 90.00, 106.52, 90.00 |
Refinement procedure
| Resolution | 48.140 - 2.920 |
| R-factor | 0.21298 |
| Rwork | 0.208 |
| R-free | 0.29762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fap |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.390 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.140 | 3.100 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Number of reflections | 23633 | 3775 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.995 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Na-HEPES buffer (pH 7.5), 20% (w/v) PEG 8000 |
