6M1K
USP7 in complex with a novel inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2019-11-13 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.065, 69.200, 76.450 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 41.180 - 2.255 |
| R-factor | 0.2351 |
| Rwork | 0.233 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n9t |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3246) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.180 | 2.336 |
| High resolution limit [Å] | 2.255 | 2.255 |
| Rmerge | 0.075 | |
| Rmeas | 0.137 | |
| Rpim | 0.074 | |
| Number of reflections | 35627 | 3451 |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 95.1 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M Tris (hydroxymethyl)aminomethane hydrochloride, pH 7.0, 20% (v/v) PEG 1000 |
