6LVL
Crystal structure of FGFR2 in complex with 1,3,5-triazine derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-12-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.675, 116.136, 64.202 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.110 - 2.980 |
| R-factor | 0.2043 |
| Rwork | 0.202 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b2t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.658 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.040 |
| High resolution limit [Å] | 2.980 | 8.110 | 2.990 |
| Rmerge | 0.119 | 0.051 | 0.357 |
| Number of reflections | 14661 | 705 | 758 |
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 87.7 | 75.5 | 91.3 |
| Redundancy | 3.9 | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 296 | Tris, Ammonium sulfate, PEG 8000 |
