6LPJ
A2AR crystallized in EROCOC17+4, LCP-SFX at 277 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2017-07-09 |
| Detector | MPCCD |
| Wavelength(s) | 1.230 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.400, 178.900, 142.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.340 - 1.800 |
| R-factor | 0.1764 |
| Rwork | 0.175 |
| R-free | 0.20710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eiy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.552 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.340 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 48530 | 2390 |
| <I/σ(I)> | 8.3 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 344.7 | 98.9 |
| CC(1/2) | 0.996 | 0.393 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM sodium citrate pH5.5, 37% PEG300, 50 mM NaSCN, and 2% 2,5-hexanediol |
