6LKQ

The Structural Basis for Inhibition of Ribosomal Translocation by Viomycin

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-04-02
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.03318
Spacegroup name	P 21 21 21
Unit cell lengths	257.600, 312.900, 328.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 3.100
Rwork	0.210
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3F1E. 3F1F
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.6)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	50.000
High resolution limit [Å]	3.100	3.100
Rmerge	0.200
Number of reflections	433307	433307
<I/σ(I)>	1.7
Completeness [%]	100.0
Redundancy	7
CC(1/2)		0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	Tris Ac PH.7.0, 25-35 mM KCL, 6.1% PEG 20000, 1% glycerol, 50mM sucrose