6LE5

Crystal structure of the mitochondrial calcium uptake 1 and 2 heterodimer (MICU1-MICU2 heterodimer) in an apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	62.970, 173.720, 148.000
Unit cell angles	90.00, 93.88, 90.00

Refinement procedure

Resolution	50.010 - 3.100
R-factor	0.29467
Rwork	0.293
R-free	0.33162
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.380
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	SHELX
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.860	3.180
High resolution limit [Å]	3.100	3.100
Number of reflections	57570	4480
<I/σ(I)>	6.2
Completeness [%]	100.0
Redundancy	7.3
CC(1/2)	0.992	0.258

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% (w/v) PEG3350 0.2 M Ammonium citrate tribasic (pH 7.5)