6LDM

Structural basis of G-quadruplex DNA recognition by the yeast telomeric protein Rap1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-09-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	84.680, 70.200, 64.850
Unit cell angles	90.00, 97.72, 90.00

Refinement procedure

Resolution	35.090 - 2.400
R-factor	0.2043
Rwork	0.203
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ign
RMSD bond length	0.009
RMSD bond angle	0.890
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.262	32.131	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge		0.030	0.406
Rmeas	0.068	0.037	0.515
Rpim	0.040	0.021	0.312
Total number of observations		1439	5258
Number of reflections	14665	480	2134
<I/σ(I)>	8.7	19.4	2
Completeness [%]	99.1	97.5	99.5
Redundancy	2.7	3	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	0.1M Sodium citrate tribasic dihydrate pH 5.6, 10% isopropanol, 14% PEG 4000, 10% of glycerol