6LAE
Crystal structure of the DNA-binding domain of human XPA in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97775 |
| Spacegroup name | P 31 |
| Unit cell lengths | 69.112, 69.112, 63.938 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.700 - 2.810 |
| R-factor | 0.2198 |
| Rwork | 0.218 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j44 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.701 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.104 | 0.495 |
| Rmeas | 0.119 | 0.562 |
| Rpim | 0.056 | 0.259 |
| Number of reflections | 7795 | 778 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 93.2 | 92.6 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 20% PEG4000, 20% 2-propanol, 0.1 mM sodium citrate tribasic pH 5.6 |
