6L6R

Crystal structure of LRP6 E1E2-SOST complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-10-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03314
Spacegroup name	P 64
Unit cell lengths	252.792, 252.792, 86.286
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.861 - 3.800
R-factor	0.2108
Rwork	0.207
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dow 2k8p
RMSD bond length	0.003
RMSD bond angle	0.726
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.861	29.860	4.010
High resolution limit [Å]	3.800	12.020	3.800
Rmerge	0.200	0.061	0.750
Rmeas	0.253	0.071	0.861
Rpim	0.120	0.034	0.407
Number of reflections	31260	979	4517
<I/σ(I)>	6.5
Completeness [%]	99.6	93.4	99.5
Redundancy	4.3	4	4.3
CC(1/2)	0.980	0.995	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		301	PEG 8000, magnesium acetate, sodium citrate, HEPES