6KUX

Crystal structures of the alpha2A adrenergic receptor in complex with an antagonist RSC.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	C 2 2 21
Unit cell lengths	71.170, 71.790, 284.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.385 - 2.700
R-factor	0.252
Rwork	0.251
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y02
RMSD bond length	0.003
RMSD bond angle	0.526
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.700	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.089	0.487
Number of reflections	18743	2155
<I/σ(I)>	9.7
Completeness [%]	91.4	73.4
Redundancy	8.1	2.5
CC(1/2)	0.999	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	0.1M Sodium citrate tribasic dihydrate pH 5.0, 290mM Ammonium chloride, 30% PEG400, 7% glycerol