6KTR

Crystal structure of fibroblast growth factor 19 in complex with Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-04-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	84.968, 104.125, 165.365
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.717 - 2.598
R-factor	0.204167575911
Rwork	0.202
R-free	0.25319
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2p23
RMSD bond length	0.003
RMSD bond angle	0.587
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	2.690
High resolution limit [Å]	2.590	2.600
Rmerge	0.017	0.145
Rmeas		0.205
Rpim	0.017	0.145
Number of reflections	45911	4486
<I/σ(I)>	3.3	3.3
Completeness [%]	99.7	99
Redundancy	2	2
CC(1/2)	0.997	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293.15	0.2M Lithium Sulfate monohydrate, 0.1M Bis-Tris pH6.9, 26% w/v Polyethylene Glycol 3350