6KTO
Crystal structure of human SHLD3-C-REV7-O-REV7-SHLD2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.843, 93.843, 325.377 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.441 - 3.450 |
| R-factor | 0.242976454103 |
| Rwork | 0.242 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vu7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.494 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.720 |
| High resolution limit [Å] | 3.440 | 3.450 |
| Rpim | 0.058 | 0.452 |
| Number of reflections | 11237 | 2312 |
| <I/σ(I)> | 12.75 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.02M Magnesium chloride hexahydrate, 0.1M HEPES 7.5, 22% w/v Poly (acrylic acid sodium salt) 5100 |
