3VU7
Crystal structure of REV1-REV7-REV3 ternary complex
Summary for 3VU7
| Entry DOI | 10.2210/pdb3vu7/pdb |
| Descriptor | DNA repair protein REV1, Mitotic spindle assembly checkpoint protein MAD2B, DNA polymerase zeta catalytic subunit (3 entities in total) |
| Functional Keywords | dna polymerase, dna replication, translesion dna synthesis, dna damage tolerance, dna repair, replication |
| Biological source | Homo sapiens (human) More |
| Cellular location | Nucleus (Probable): Q9UBZ9 Nucleus: Q9UI95 Nucleus (Potential): O60673 |
| Total number of polymer chains | 3 |
| Total formula weight | 45828.65 |
| Authors | Kikuchi, S.,Hara, K.,Shimizu, T.,Sato, M.,Hashimoto, H. (deposition date: 2012-06-20, release date: 2012-08-08, Last modification date: 2023-11-08) |
| Primary citation | Kikuchi, S.,Hara, K.,Shimizu, T.,Sato, M.,Hashimoto, H. Structural basis of recruitment of DNA polymerase [zeta] by interaction between REV1 and REV7 proteins J.Biol.Chem., 287:33847-33852, 2012 Cited by PubMed Abstract: REV1, REV3, and REV7 are pivotal proteins in translesion DNA synthesis, which allows DNA synthesis even in the presence of DNA damage. REV1 and REV3 are error-prone DNA polymerases and function as inserter and extender polymerases in this process, respectively. REV7 interacts with both REV1 and REV3, acting as an adaptor that functionally links the two, although the structural basis of this collaboration remains unclear. Here, we show the crystal structure of the ternary complex, composed of the C-terminal domain of human REV1, REV7, and a REV3 fragment. The REV1 C-terminal domain adopts a four-helix bundle that interacts with REV7. A linker region between helices 2 and 3, which is conserved among mammals, interacts with the β-sheet of REV7. Remarkably, the REV7-binding interface is distinct from the binding site of DNA polymerase η or κ. Thus, the REV1 C-terminal domain might facilitate polymerase switching by providing a scaffold for both inserter and extender polymerases to bind. Our structure reveals the basis of DNA polymerase ζ (a complex of REV3 and REV7) recruitment to the stalled replication fork and provides insight into the mechanism of polymerase switching. PubMed: 22859296DOI: 10.1074/jbc.M112.396838 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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